rat mab anti mouse cd206  (Bio-Rad)

Journal: EMBO Molecular Medicine

Article Title: CXCR4 engagement triggers CD47 internalization and antitumor immunization in a mouse model of mesothelioma

doi: 10.15252/emmm.202012344

Figure Lengend Snippet: Representative IHC staining for F4/80, CD45R, CD3, and HMGB1 in two mouse mesotheliomas. Nuclei were counterstained with hematoxylin. Scale bar 20 µm. Representative immunofluorescence (IF) staining for F4/80 (red), CD86 (green, in the upper panel), and CD206 (green, in the lower panel) of mouse MM lesions. Scale bar 50 µm. Representative IHC staining for CD68, CD206, CD163, CD20, CD3, and HMGB1 in human sarcomatoid and epithelioid mesotheliomas. Scale bar 50 µm.

Article Snippet: For immunofluorescent staining, 6‐μm‐thick serial cryostat sections from mice tumor samples, immediately snap‐frozen in OCT after removal, were fixed with cold acetone for 10 min and co‐stained with the following Ab: rabbit anti‐mouse mAb to F4/80 (1:100, Biolegend), rat anti‐mouse mAb to CD206 (1:200 Bio‐Rad), and rat anti‐mouse mAb to CD86 (clone PO.3, 1:100, #04‐1527 Millipore).

Techniques: Immunohistochemistry, Immunofluorescence, Staining

Journal: Cancers

Article Title: Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection

doi: 10.3390/cancers12030717

Figure Lengend Snippet: Quantification of different key markers of tumor infiltrating immune cells, as follows: CD86 + M1 and CD206 + M2 (panel A ), Gr-1 (GR) for granulocytes and CD11b + Gr-1 + for MDSCs (panel B ), CD31 for vessels (panel C ), and CD4 and CD8 for T cells (panel D ) per HMMF in BALB/c mice injected with C51 P, C51 E, and C51 FL Rnaset2 cell lines ( N = six animals in group). The mean ± SE are indicated. Immunohistochemistry and immunofluorescence analyses of tumor sections from BALB/c mice injected with C51 P, C51 E, and C51 FL Rnaset2 cell lines at indicated time points are shown. An ANOVA statistical analysis was performed assuming p < 0.05 as a threshold value. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Afterward, cells were labeled with anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7), and anti-CD11b (clone M1/70) mAbs, all from eBioscience (San Diego, CA, USA); anti-F4/80 (clone BM8) and anti-CD86 (clone GL-1) mAbs from BioLegend (San Diego, CA, USA), and anti-CD206 (clone MR5D3) mAb from BIO-RAD.

Techniques: Injection, Immunohistochemistry, Immunofluorescence

Journal: Cancers

Article Title: Overexpression of Murine Rnaset2 in a Colon Syngeneic Mouse Carcinoma Model Leads to Rebalance of Intra-Tumor M1/M2 Macrophage Ratio, Activation of T Cells, Delayed Tumor Growth, and Rejection

doi: 10.3390/cancers12030717

Figure Lengend Snippet: Cytometric flow assessment after in vitro tumor digestion has been performed to study percentage of intra-tumor CD45 + CD86 + M1 and CD206 + M2 macrophage ratio in BALB/c mice ( N = three animals in group) injected with C51 P, C51 E and C51 FL Rnaset2 cell lines (panel A ); gating strategy for flow cytometry (FACS) analysis of surface markers in M1 (F4/80 + CD86 + ) and M2 (F4/80 + CD206 + ) macrophages (panel B ). An ANOVA statistical analysis was performed assuming p < 0.05 as a threshold value. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Afterward, cells were labeled with anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7), and anti-CD11b (clone M1/70) mAbs, all from eBioscience (San Diego, CA, USA); anti-F4/80 (clone BM8) and anti-CD86 (clone GL-1) mAbs from BioLegend (San Diego, CA, USA), and anti-CD206 (clone MR5D3) mAb from BIO-RAD.

Techniques: In Vitro, Injection, Flow Cytometry

Journal: Life Science Alliance

Article Title: The role of MHC class I recycling and Arf6 in cross-presentation by murine dendritic cells

doi: 10.26508/lsa.201900464

Figure Lengend Snippet: (A) Transduced C57BL/6 BM-DCs were pulsed with various amounts of SL8 peptide, fixed, and cocultured with OT-I T cells. The next day, the supernatant was recovered and probed for IL-2 by ELISA. (B, C, D, E, F) Cross-presentation to OT-I cells of peptide GS-20 (B), of irradiated yeast cells expressing full length OVA at the cell surface (C), of OVA-anti-OVA IC (D), and of the OVA fusion protein P3UO targeted to CD11c (E), or CD206 (F). (A, B, C, D, E, F) Data represent the means and SD of n = 3 independent experiments (A, B, C, E, F) and n = 4 (D). Data were evaluated with a one-sample t test, under the null hypotheses that the column means of the sample are equal to 100%. Data are significantly different if P ≤ 0.05 (*), P ≤ 0.01 (**), or P ≤ 0.001 (***). NT, nontargeting shRNA.

Article Snippet: MAb rat anti-mouse CD206 (clone MR5D3) was from Bio-Rad.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation, Expressing, IF-P, shRNA

Journal: Life Science Alliance

Article Title: The role of MHC class I recycling and Arf6 in cross-presentation by murine dendritic cells

doi: 10.26508/lsa.201900464

Figure Lengend Snippet: Cells were treated as on . (A, B, C, D, E, F) , SL8 peptide (B), GS-20 long peptide (C), OVA-yeast (D), IC anti ovalbumin–ovalbumin (E), fusion protein P3UO targeted to CD11c (F), fusion protein P3UO targeted to CD206. Data represent a single experiment with mean and SD of two duplicate samples. NT, nontargeting shRNA.

Article Snippet: MAb rat anti-mouse CD206 (clone MR5D3) was from Bio-Rad.

Techniques: shRNA